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Hypervariable allelic expression patterns of the imprinted IGF2 gene in tumor cells

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

He, LM, Cui, HM, Walsh, Colum, Mattsson, R, Lin, WL, Anneren, G, Pfeifer-Ohlsson, S and Ohlsson, R (1998) Hypervariable allelic expression patterns of the imprinted IGF2 gene in tumor cells. ONCOGENE, 16 (1). pp. 113-119. [Journal article]

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DOI: 10.1038/sj.onc.1201501

Abstract

The IGF2 gene, which encodes a growth factor, is subject to genomic imprinting, The frequently observed loss of IGF2 imprinting in a variety of tumors has been suggested to contribute to neoplasia, Since these reports have not documented the imprinting status of IGF2 at the cellular level, it cannot be excluded that the imprinting status might vary within the tumor, The possibility that loss of IGF2 imprinting in neoplastic cells reflects random imprinting patterns, was therefore addressed, We show here that individual cell populations of the JEG-3 choriocarcinoma cell line display heterogenous imprinting patterns of both IGF2 and H19, In addition, a lack of correlation between IGF2 and H19 imprinting status suggests that any regional parental imprint has been functionally lost, This notion is reinforced by the observation that JEG-3 cell subclones display a range of promoter-specific IGF2 allele usage, Moreover, we observed that the imprinting status of H19 and IGF2 were differentially modulated in JEG-3-derived tumors generated in nude mice, The results suggest that allele-specific expression of IGF2 operates in the absence of a parental imprint, Finally, our observations urge caution with respect to the general interpretation of biallelic expression as `loss of imprinting'.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:21699
Deposited By:Professor Colum Walsh
Deposited On:02 Apr 2012 11:03
Last Modified:11 Dec 2012 14:44

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