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A rapid in situ, colorimetric assay for the determination of mammalian cell viability in alginate-immobilized and encapsulated systems

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Rollan, A, McCormack, D, McCormack, H, McHale, L and McHale, AP (1996) A rapid in situ, colorimetric assay for the determination of mammalian cell viability in alginate-immobilized and encapsulated systems. BIOPROCESS ENGINEERING, 15 (1). pp. 47-49. [Journal article]

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DOI: 10.1007/BF00435528

Abstract

A convenient, rapid colorimetric assay system has been developed in order to determine cell viability of populations of mammalian cells encapsulated using a poly-L-lysine/alginate encapsulation system. The method is based on the use of 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and involves direct incubation of the capsules with the reagent. This is followed by direct solubilization of the resulting formazan product in dimethyl sulfoxide (DMSO). In order to demonstrate that this assay method may be generally applicable to alginate-based carrier systems, viability of cells immobilized in alginate beads, as distinct from encapsulation in the poly-L-lysine/alginate system, was also determined using the in situ method.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:21527
Deposited By:Professor Anthony McHale
Deposited On:28 Mar 2012 16:21
Last Modified:04 Dec 2012 11:51

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