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STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

IRHUMA, A, GALLAGHER, J, HACKETT, TJ and MCHALE, AP (1991) STUDIES ON N-ACETYLGLUCOSAMINIDASE ACTIVITY PRODUCED BY STREPTOMYCES-HYGROSCOPICUS. BIOCHIMICA ET BIOPHYSICA ACTA, 1074 (1). pp. 1-5. [Journal article]

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DOI: 10.1016/0304-4165(91)90029-G

Abstract

Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucoseaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30-degrees-C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:21451
Deposited By:Professor Anthony McHale
Deposited On:28 Mar 2012 16:32
Last Modified:04 Dec 2012 12:03

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