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MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

BUNNI, L, HACKETT, TJ, MCHALE, L, FLYNN, G and McHale, Anthony (1993) MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A TALAROMYCES-EMERSONII DERIVED ALPHA-AMYLASE ENCODING GENETIC DETERMINANT IN A HUMAN CELL-LINE. BIOTECHNOLOGY LETTERS, 15 (11). pp. 1095-1100. [Journal article]

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DOI: 10.1007/BF00131196

Abstract

A cDNA fragment encoding a Talaromyces emersonii acid stable alpha-amylase has, been cloned into a mammalian cell expression vector system. When human HeLa cells were transformed with this DNA, functional enzyme could be detected in extracellular media and cell lysates derived from stable transformants. Expression of the T. emersonii derived cDNA was verified by northern dot blotting hybridisation analysis of mRNA extracted from transformants, using the labelled amylase encoding cDNA determinant as a probe. Results obtained suggest that expression of the fungus derived alpha-amylase in the transformed HeLa cells is glucocorticoid dependent.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:21449
Deposited By:Professor Anthony McHale
Deposited On:28 Mar 2012 16:31
Last Modified:04 Dec 2012 12:02

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