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Unique structural features of interconverting monomeric and dimeric G-quadruplexes adopted by a sequence from intron of N-myc gene

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Trajkovski, Marko, Webba da Silva, Mateus and Plavec, Janez (2012) Unique structural features of interconverting monomeric and dimeric G-quadruplexes adopted by a sequence from intron of N-myc gene. Journal of the American Chemical Society, 134 . [Journal article]

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URL: http://pubs.acs.org/doi/abs/10.1021/ja208483v?prevSearch=webba&searchHistoryKey=

DOI: 10.1021/ja208483v

Abstract

Multidimensional heteronuclear NMR study has demonstrated that a guanine-rich DNA oligonucleotide originating from N-myc gene folds into G-quadruplex structures in the presence of K+, NH4+ and Na+ ions. A monomeric G-quadruplex formed in K+ ion containing solution exhibits three G- quartets and flexible propeller-type loops. The 3D structure with three single nucleotide loops represents a missing element in structures of parallel G-quadruplexes. The structural features together with the high temperature stability are suggestive of specific biological role of G-quadruplex formation within intron of N-myc gene. Increase in K+ ion and oligonucleotide concentrations resulted in transformation of monomeric G-quadruplex into a dimeric form. The dimeric G-quadruplex exhibits six stacked G- quartets, parallel strand orientations and propeller-type loops. A link between the third and the fourth G- quartets consists of two adenine residues that are flipped out to facilitate consecutive stacking of six G- quartets.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:20983
Deposited By:Dr Mateus Webba da Silva
Deposited On:23 Feb 2012 11:31
Last Modified:04 Dec 2012 14:19

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