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Topological Characterization of G-Quadruplexes by UV Absorption and Circular Dichroism

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Karsisiotis, Ioannis, Ma’ani Hessari, N, Novellino, E, Spada, GP, Randazzo, A and Webba da Silva, Mateus (2011) Topological Characterization of G-Quadruplexes by UV Absorption and Circular Dichroism. Angewandte Chemie International Edition, 50 . pp. 10645-10648. [Journal article]

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URL: http://onlinelibrary.wiley.com/doi/10.1002/anie.201105193/abstract

DOI: 10.1002/anie.201105193

Abstract

The emergence of nucleic acid four-stranded architectures, denominated G-quadruplexes as a prolific area of research, has led to an interest in the development of inexpensive methods for the rapid assessment of their structural characterization in solution. Research in this area is motivated by their potential impact in regulation of biological mechanisms and technological applications. For many applications, light absorption techniques, such as circular dichroism (CD) and UV, have been sufficient to discriminate the quadruplex fold from other architectures. CD is also useful to discriminate a single quadruplex topology from all other 25 generic folding topologies. Here we demonstrate the use of these techniques for characterizing three different types of G-quadruplex topologies classified through the sequence of glycosidic bond angles (GBA) adopted by guanosines of the G-quadruplex stem.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:20458
Deposited By:Dr Mateus Webba da Silva
Deposited On:09 Nov 2011 14:13
Last Modified:26 Nov 2012 11:38

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