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A NONISOTOPIC DNA HYBRIDIZATION ASSAY FOR THE IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS ISOLATED FROM FOODS

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Wilson, IG, Gilmour, K, Cooper, JE, Bjourson, AJ and Harvey, J (1994) A NONISOTOPIC DNA HYBRIDIZATION ASSAY FOR THE IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS ISOLATED FROM FOODS. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 22 (1). pp. 43-54. [Journal article]

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Abstract

A digoxygenin-labelled total genomic DNA probe was used to identify Staphylococcus aureus. Isolation and identification of organisms was possible in less than 4 days. Identification alone could be completed in less than 2 days, compared with over 5 days for identification of isolates by multipoint inoculation. The probe showed excellent discrimination of S. aureus from other staphylococci and from a wide range of bacteria commonly associated with milk and meat. The effectiveness of this probe was tested against cultural isolation of staphylococci in milk using Baird-Parker agar followed by identification using multipoint inoculation and API Staph. The probe gave comparable results to the conventional methods and, for large sample numbers, offered lower cost and greater ease of use.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:1950
Deposited By:Mrs Caroline Adams
Deposited On:07 Dec 2009 11:42
Last Modified:14 Oct 2013 15:51

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