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Temporal and spatial expression analyses of TrEhod40, TrEnod5 and a novel early nodulin in white clover roots and nodules

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Crockard, MA, Bjourson, AJ, Pulvirenti, MG and Cooper, JE (2001) Temporal and spatial expression analyses of TrEhod40, TrEnod5 and a novel early nodulin in white clover roots and nodules. PLANT SCIENCE, 161 (6). pp. 1161-1170. [Journal article]

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URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TBH-448Y0P3-H&_user=126978&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000010438&_version=1&_urlVersion=0&_userid=126978&md5=0f8abb64696365fabbabc0a25bc6511f

Abstract

Using differential display RT-PCR on mRNA populations from white clover roots challenged with a mock-inoculation or the microsymbiont, Rhizobium leguminosarum biovar trifolii ANU843, a number of expressed sequence tags (ESTs), specific to the early stages of symbiosis, were identified. Similarity searches of many of these ESTs identified them as novel, with no significant sequence homologues. Of the white clover cDNA fragments with homologies to sequences in EMBL databases, several early nodulins were identified and further characterized by RT-PCR and in situ hybridization analyses to provide detailed temporal and spatial expression profiles. Here, we report on the analyses of a clover Enod40 homologue and a new early nodulin precursor (DD17), with 3' homology to Enod8, previously only identified in Medicago species. An arabinogalactan-like protein, highly homologous to previously reported Enod5s from other legumes. was also characterized and used as a standard for all expression analyses, following its isolation through degenerate-primer RT-PCR. Published by Elsevier Science Ireland Ltd.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:1939
Deposited By:Mrs Caroline Adams
Deposited On:30 Nov 2009 09:38
Last Modified:14 Oct 2013 15:35

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