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Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Chen, TB, Orr, DF, Bjourson, AJ, McClean, Stephen, O'Rourke, M, Hirst, DG, Rao, PF and Shaw, C (2002) Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 (18). pp. 4693-4700. [Journal article]

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DOI: 10.1046/j.1432-1033.2002.03174.x

Abstract

Two novel bradykinin-related peptides (Ala3,Thr6)-bradykinin and (Val1,Thr3,Thr6)-bradykinin, were identified by a systematic sequencing study of peptides in the defensive skin secretion of the yellow-bellied toad, Bombina variegata . These peptides are the first amphibian skin bradykinins to exhibit amino acid substitutions at the Pro3 position of the bradykinin nonapeptide. Previously reported bradykinins from other Bombina species were not detected. Respective precursor cDNAs, designated BVK-1 and BVK-2, respectively, were cloned from a skin library by 3'- and 5'-RACE reactions. BVK-1 contained an open-reading frame of 97 amino acids encoding a single copy of (Ala3,Thr6)-bradykinin and similarly, the open-reading frame of BVK-2 consisted of 96 amino acids encoding a single copy of (Val1,Thr3,Thr6)-bradykinin. Synthetic replicates of each novel bradykinin were found to be active on mammalian arterial and small intestinal smooth muscle preparations. The structural diversity of bradykinins in amphibian defensive skin secretions may be related to defence against specific predators.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:1937
Deposited By:Mrs Caroline Adams
Deposited On:30 Nov 2009 09:18
Last Modified:14 Oct 2013 15:33

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