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Amphibian skin secretomics: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea: Discovery of a novel peptide family, the hyposins

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Thompson, Alan Hunter, Bjourson, AJ, Orr, David Francis, Shaw, Chris and McClean, Stephen (2007) Amphibian skin secretomics: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea: Discovery of a novel peptide family, the hyposins. JOURNAL OF PROTEOME RESEARCH, 6 (9). pp. 3604-3613. [Journal article]

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DOI: 10.1021/pr0702666

Abstract

This study reports the variety of peptides present in the skin secretory peptidome of Phyllomedusa hypochondrialis azurea. Peptide structures, along with post-translational modifications, were elucidated by QTOF MS/MS analysis, cDNA sequencing, or a combination of both. Twenty-two peptides, including 19 novel structures, were identified from six different structural classes, including tryptophyllins, dermorphins, and a novel group of peptides termed hyposins. The study demonstrates the power of this combined approach to mine the rich peptidome compliment of the amphibian defensive skin secretome.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:1923
Deposited By:Mrs Caroline Adams
Deposited On:30 Nov 2009 09:20
Last Modified:14 Oct 2013 15:24

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