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Association of DNA Quadruplexes through G:C:G:C Tetrads. Solution Structure ofd(GCGGTGGAT)

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Webba da Silva, Mateus (2003) Association of DNA Quadruplexes through G:C:G:C Tetrads. Solution Structure ofd(GCGGTGGAT). Biochemistry, 42 . [Journal article]

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DOI: 10.1021/bi0355185

Abstract

The structure formed by the DNA sequence d(GCGGTGGAT) in a 100 mM Na+ solution hasbeen determined using molecular dynamics calculations constrained by distance and dihedral restraintsderived from NMR experiments performed at isotopic natural abundance. The sequence folds into a dimerof dimers. Each symmetry-related half contains two parallel stranded G:G:G:G tetrads flanked by an A:Amismatch and by four-stranded G:C:G:C tetrads. Each of the two juxtaposed G:C:G:C tetrads is composedof alternating antiparallel strands from the two halves of the dimer. For each single strand, a thymineintersperses a double chain reversal connecting the juxtaposed G:G:G:G tetrads. This architecture haspotential implications in genetic recombination. It suggests a pathway for oligomerization involvingassociation of quadruplex entities through GpC steps.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:18300
Deposited By:Dr Mateus Webba da Silva
Deposited On:03 May 2011 10:48
Last Modified:04 Dec 2012 14:30

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