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Kinetics of double-chain reversals bridgingcontiguous quartets in tetramolecular quadruplexes

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Mergny, J.L., De Cian, A, Amrane, S and Webba da Silva, Mateus (2006) Kinetics of double-chain reversals bridgingcontiguous quartets in tetramolecular quadruplexes. Nucleic Acids Research, 34 . [Journal article]

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DOI: 10.1093/nar/gkl098

Abstract

Repetitive 50GGXGG DNA segments abound in, ornear, regulatory regions of the genome and mayform unusual structures called G-quadruplexes.Using NMR spectroscopy, we demonstrate that afamily of 50GCGGXGGY sequences adopts a foldingtopology containing double-chain reversals. Thetopology is composed of two bistranded quadruplexmonomeric units linked by formation of G:C:G:Ctetrads. We provide a complete thermodynamic andkinetic analysis of 13 different sequences usingabsorbance spectroscopy and DSC, and comparetheir kinetics with a canonical tetrameric parallelstrandedquadruplex formed by TG4T. We demonstratelarge differences (up to 105-fold) in the associationconstants of these quadruplexes depending onprimary sequence; the fastest samples exhibitingassociation rate equal or higher than the canonicalTG4T quadruplex. In contrast, all sequences studiedhere unfold at a lower temperature than this quadruplex.Some sequences have thermodynamic stabilitycomparable to the canonical TG4T tetramolecularquadruplex, but with faster association and dissociation.Sequence effects on the dissociation processesare discussed in light of structural data.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:18294
Deposited By:Dr Mateus Webba da Silva
Deposited On:03 May 2011 11:15
Last Modified:04 Dec 2012 14:24

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