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Effect of Base Sequence on G-Wire Formation in Solution

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Spindler, L, Rigler, R, Drevenˇsek-Olenik, I, Ma’ani Hessari, N and Webba da Silva, Mateus (2010) Effect of Base Sequence on G-Wire Formation in Solution. Journal of Nucleic Acids, 2010 (Article ID 431651,). [Journal article]

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DOI: doi:10.4061/2010/431651

Abstract

The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamiclight scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, westudied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS andPAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly andconsequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, whilesome discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longestmacromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentrationdependence in the investigated DNA concentration range (0.1 mM–0.4mM) and no structural change upon heating.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:18290
Deposited By:Dr Mateus Webba da Silva
Deposited On:03 May 2011 11:26
Last Modified:04 Dec 2012 14:22

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