Abstract

Guanine-rich sequences of nucleic acids may fold into secondarystructural folds called quadruplex architectures.These architectures are a rapidly growing theme of interestwith promising repercussions in our understanding of biologyand practical applications in medical fields, materialsscience, and biotechnology. Currently, there is thus interestin determining both topology and their atomic detail 3Dstructures. The establishment of solution conditions suitablefor structural studies of G-quadruplex architectures requiresthe determination of the level of oligomerization (stoichiometry)of DNA strands. Various analytical techniques arecurrently applied for the routine assessment of the stoichiometrythat generally include conditions not representativeof the environment in which the structural studies are performed.