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Study of immobilized yeast, Saccharomyces cerevisiae, using flow microcalorimetry.

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Owusu, Richard and Finch, Arthur (1985) Study of immobilized yeast, Saccharomyces cerevisiae, using flow microcalorimetry. The Journal of General and Applied Microbiology, 31 (3). pp. 221-230. [Journal article]

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URL: http://dx.doi.org/10.2323/jgam.31.221

DOI: doi:10.2323/jgam.31.221


When immobilized yeast was exposed to nutrients, the resulting heat effect (dQ/dt; J sec-1) increased exponentially with a doubling time (t2) of 2.2±0.3hr. The half life (dQ/dt) under non-growing conditions with sucrose as substrate was 84hr. The kinetics of the transformation of a series of sugars were characterised. The Michaelis constant (Km) and maximal heat effect, (dQ/dt)max, were determined using two common enzyme kinetics linearization plots. The shapes of the Eadie plots for some sugars are discussed in terms of currently proposed mechanisms of their uptake

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:17619
Deposited By:Dr Richard Owusu-Apenten
Deposited On:01 Apr 2011 10:01
Last Modified:01 Apr 2011 10:01

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