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Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Trewhella, Martin, Owusu, Richard and Finch, Arthur (1985) Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 830 (3). pp. 282-287. [Journal article]

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URL: http://dx.doi.org/10.1016/0167-4838(85)90284-5

DOI: doi:10.1016/0167-4838(85)90284-5

Abstract

The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:17399
Deposited By:Dr Richard Owusu-Apenten
Deposited On:03 Mar 2011 14:06
Last Modified:03 Mar 2011 14:06

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