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Muscarinic Acetylcholine Receptor-Mediated Phosphoinositide Turnover in Cultured Human Retinal Pigment Epithelium Cells.

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Osborne, NN, FitzGibbon, F and Schwartz, G (1991) Muscarinic Acetylcholine Receptor-Mediated Phosphoinositide Turnover in Cultured Human Retinal Pigment Epithelium Cells. Vision Research, 31 (7-8). pp. 1119-1127. [Journal article]

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URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T0W-484DYYV-1C8&_user=126978&_coverDate=12%2F31%2F1991&_alid=1459412607&_rdoc=10&_fmt=high&_orig=search&_origin=search&_zone=rslt_list_item&_cdi=4873&_sort=r&_st=13&_docanchor=&view=c&_ct=16&_acct

DOI: 10.101`6/0042-6989(91)90038-7

Abstract

Cultured retinal pigment epithelium cells prepared from post-mortem adult human eyes are shown to contain muscarinic receptors associated with phosphoinositide turnover. Carbachol at a concentration of 100μM induced a four-fold increase in3H-inositol phosphates (more than 76% is in the form of3H-inositol-1-phosphate) accumulation within 45 min in cells prelabelled with3H-myoinositol and exposed to 5mM LiCl. The EC50 of carbachol was approx. 70μM and the saturation concentration was about 1 mM. The carbachol-induced response was blocked by both atropine and pirenzepine, the former being most effective. Pre-exposure of cells to carbachol resulted in desensitization and a drastic reduction in the subsequent carbachol-induced stimulation of3H-inositol phosphates. The carbachol response could be attenuated by the biologically active phorbol ester, 4β-phorbol 12-myristate 13-acetate, and this was nullified by the protein kinase C inhibitor, staurosporine. The biologically inactive phorbol ester, 4α-phorbol 12,13 dideconoate, did not attenuate the carbachol-induced stimulation of3H-inositol phosphates. Pertussis toxin failed to influence the carbachol receptor-mediated phosphoinositide turnover. These studies provide clear evidence for the occurrence of muscarinic receptors coupled to phosphoinositide hydrolysis on human retinal pigment epithelium cells.

Item Type:Journal article
Keywords:Phosphoinositide turnover;pigment epithelium cells;cholinergic receptors
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:15629
Deposited By:Mr Francis FitzGibbon
Deposited On:16 Sep 2010 14:46
Last Modified:04 Oct 2010 12:22

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