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Metabolic Interactions of Glucose, Acetoacetate and Insulin in Mammary- Glad Slices of Lactating Rats

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Williamson, DH, McKeown, SR and Ilic, V (1975) Metabolic Interactions of Glucose, Acetoacetate and Insulin in Mammary- Glad Slices of Lactating Rats. Biochemical Journal, 150 (2). pp. 145-152. [Journal article]

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Abstract

1.Utilisation of 5mM- glucose by slices of lactating mammary gland was decreased 33% on addition of acetoacetate (2mM) to the incubation medium. This inhabitation was accompanied by increases in the intracellular concentrations if citrate and glucose 6-phosphate. 2.In the presence of acetoacetate the accumulation of pyruvate in the medium approximately doubled. 3.Insulin completely reversed the inhibitory effect of acetoacetate on glucose utilisation, without altering the amount of acetoacetate removed or pyruvate formed. 4.Similar results were obtained with mammary -gland slices from diabetic rats, except that insulin did not completely reverse the effects of the acetoacetate. 5. Acetoacetate inhibited the formation of 14CO2 [1-14C]pryuvate; this effect was not overcome by insulin. 6. Insulin increased the proportion of [3-14C] acetoacetate that was converted into lipid and decreased that oxidized to CO2. 7. The physiological significance of these findings is discussed.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Health Sciences
Research Institutes and Groups:Institute of Nursing and Health Research > Centre for Health and Rehabilitation Technologies
ID Code:15104
Deposited By:Miss Anna Mooney
Deposited On:24 Aug 2010 09:04
Last Modified:01 Mar 2012 10:52

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