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Stabilization and partial purification of a protease from ginger rhizome (Zingiber offinale Roscoe)

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Adulyatham, Pitiya and Owusu-Apenten, Richard (2005) Stabilization and partial purification of a protease from ginger rhizome (Zingiber offinale Roscoe). Journal of Food Science, 70 (3). C231-C234. [Journal article]

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Abstract

Ginger protease (GP) or zingibain is of interest as a meat tenderizing agent. The objective of this research was to investigate food-compatible methods for stabilizing GP during storage or enzyme fractionation. Crude GP extracted from fresh ginger had a half-life (t(1/2)) of 2.1 (+/- 0.16) d at 5 degrees C decreasing to 20 min at 30 degrees C. Addition of ascorbate (0.2% w/v) increased the t(1/2) for GP from 2 to 20 d at 5 degrees C. Dithiothreitol or Ethylenediaminetetraacetic acid (EDTA) had no effect on GP stability. Acetone powder preparations from ginger yielded GP with t(1/2) of 18 mo at 5 degrees C. Crude GP extracted from acetone powder was sufficiently stabilized to allow fractionation by ion exchange chromatography without the addition of toxic or expensive additives. GP was partially purified 252-fold with a recovery of 61%. The nomimal molecular weight of GP was 34.8 kDa compared with 25.1 kDa for papain. This work shows that the stability of GP can be greatly improved, increasing its attractiveness as a commercial product. Some possible routes of GP deactivation and stabilization are discussed.

Item Type:Journal article
Keywords:ginger protease; zingibain; stabilization; purification; formulations; ascorbate, plant proteases, proteins
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:14957
Deposited By:Dr Richard Owusu-Apenten
Deposited On:17 Aug 2010 11:27
Last Modified:28 Jan 2014 14:46

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