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Electrochemical detection of thiols in biological media

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Lawrence, NS, Davis, J and Compton, RG (2001) Electrochemical detection of thiols in biological media. TALANTA, 53 (5). pp. 1089-1094. [Journal article]

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Abstract

The utilisation of catechol as an electrochemical indicator fur the presence of sulphydryl thiols (RSH) has been investigated. The electrochemical oxidation of the catechol within tissue culture media was examined with the influence exerted on the redox chemistry by cysteine evaluated in terms of the development of an analytical protocol. The electro-generation of o-quinone was found to be followed by a 1,4-addition reaction with available cysteine such that an increase in the current, attributed to the re-oxidation of the thiol-catechol adduct, could be exploited as means of quantifying the concentration of the thiol. The selectivity of the reaction has been assessed with no interference from lysine, tyrosine, methionine or cystine. Other amino acids possessing sulphydryl thiol functionalities (homocysteine and glutathione) were, however, found to react through a similar route to that observed with cysteine. (C) 2001 Elsevier Science B.V. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:14381
Deposited By:Professor James Davis
Deposited On:01 Jul 2010 09:56
Last Modified:18 Aug 2011 11:18

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