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Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Hignett, G, Threlfell, S, Wain, AJ, Lawrence, NS, Wilkins, SJ, Davis, J, Compton, RG and Cardosi, MF (2001) Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine. ANALYST, 126 (3). pp. 353-357. [Journal article]

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Abstract

Square wave voltammetry was applied to the detection of cysteine through the use of an indirect assay that exploits the reaction of the thiol with a quinone indicator. Voltammetric discrimination between unreacted quinone and the corresponding quinone-cysteine adduct is possible with clear resolution of the latter peak providing a linear response from 5 to 47 muM. The selectivity of the approach was assessed with no interference from cystine, lysine, paracetamol or 4-aminophenol. The response recorded in the presence of a massive excess of ascorbic acid was also investigated and the integrity of the approach confirmed. The effects of other sulfhydryl thiols, homocysteine and glutathione, were also assessed and found to present no appreciable change in the voltammetric profile. The practical utility of the approach was investigated through examining the response to cysteine in urine.

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:14379
Deposited By:Professor James Davis
Deposited On:01 Jul 2010 09:55
Last Modified:18 Aug 2011 11:18

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