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An electrochemical adaptation of Ellman's test

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Nekrassova, O, White, PC, Threlfell, S, Hignett, G, Wain, AJ, Lawrence, NS, Davis, J and Compton, RG (2002) An electrochemical adaptation of Ellman's test. ANALYST, 127 (6). pp. 797-802. [Journal article]

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DOI: 10.1039/b202780c

Abstract

The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 muM, a limit of detection of 1.17 muM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:14358
Deposited By:Professor James Davis
Deposited On:01 Jul 2010 09:33
Last Modified:18 Aug 2011 11:19

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