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Application of a picrate semi-quantitative screening assay for the cyanogenic potential of cassava roots at a remote field site

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Wilson, WM, O'Brien, Gerard and Dufour, DL (2000) Application of a picrate semi-quantitative screening assay for the cyanogenic potential of cassava roots at a remote field site. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 80 (5). pp. 590-594. [Journal article]

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Abstract

The purpose of this research was to test the accuracy of the picrate screening assay (PSA) in the evaluation of the cyanogenic potential of cassava roots at a remote held site under conditions of unusual difficulty. To do this, a PSA was conducted in the field and compared to data collect-ed previously on five of the same cassava varieties using the Cooke colorimetric enzymic assay. PSA data were collected for 10 different cassava varieties in the Tukanoan Indian village of Vapu Tn the Colombian Amazon region. The PSA results agree with Tukanoan classifications of cassava; that Is, those classified as `sweet' by the Tukanoans generally had low-to-moderate parenchymal cyanogenic potential (0-50 mg kg(-1) fwb of HCN), while those that they classify as `bitter' had high parenchymal cyanogenic potential (greater than or equal to 100 mg kg(-1) fwb of HCN). The PSA results also agree with the data collect-ed using the Cooke colorimetric enzymic assay. The data suggest that the PSA is an appropriate test of the cyanogenic potential of cassava roots in remote field sites. (C) 2000 Society of Chemical Industry.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:14198
Deposited By:Mrs Alison Deehan
Deposited On:15 Jun 2010 09:23
Last Modified:01 Nov 2011 10:40

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