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Initial evaluation of a field-friendly extraction procedure for the enzymatic assay of cassava cyanogens

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

O'Brien, Gerard M., Chow, Emily P. L. and Price, Ruth K. (2007) Initial evaluation of a field-friendly extraction procedure for the enzymatic assay of cassava cyanogens. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 42 (8). pp. 999-1006. [Journal article]

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DOI: 10.1111/j.1365-2321.2006.01396.x


A novel `field friendly' extraction procedure has been developed for the enzymatic colorimetric determination of cyanogenic potential (CNP) in fresh cassava root parenchyma. The novel procedure does not require electrical power or vacuum, and employs inexpensive lightweight equipment, making it suitable for remote field sites. Testing of the procedure involved ten fresh roots (24-80 mg kg(-1) total CNP, as HCN, fresh basis). From the parenchyma of each root, one extract was made using the novel procedure, and a `control' extract was made using a traditional laboratory-based procedure. Total CNP assay of the extracts indicated strong (y = mx) or very strong (y = mx + c) correlation of results obtained using the two procedures, while a very strong correlation (y = mx) was obtained for free HCN. Based on this preliminary evidence, the novel procedure is satisfactory at least for total CNP assay of fresh low-CNP cassava roots.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:14194
Deposited By:Mrs Alison Deehan
Deposited On:15 Jun 2010 09:23
Last Modified:01 Nov 2011 12:30

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