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Approaching intelligent infection diagnostics: Carbon fibre sensor forelectrochemical pyocyanin detection

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Sharp, D, Gladsrone, P, Smith, RB, Forsythe, SJ and Davis, J (2010) Approaching intelligent infection diagnostics: Carbon fibre sensor forelectrochemical pyocyanin detection. Bioelectrochemistry, 77 . pp. 114-119. [Journal article]

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Abstract

Pyocyanin is produced by Ps. aeruginosa as a result of quorum sensing during wound colonisation increasing bacterial virulence and damaging host physiology, both of which contribute to an increased risk of infection. The use of carbon fibre tow as an electrochemical sensing matrix for assessing pyocyanin production is evaluated. Prototype sensor assemblies have been developed and response characteristics towards pyocyaninare detailed. The sensitive and linear quantification of pyocyanin is presented (r2=0.998) across the biomedically relevant concentration range (1–100 μM). Precise electrochemical measurements of pyocyanin by square wave voltammetry are established using carbon fibre assemblies (coefficient of variance=1.2 and 1.4% for 10 and 50 μM pyocyanin, respectively). Further testing of the sensors in bacterial cultures shows the ability to monitor pyocyanin production by Ps. aeruginosa in agreement with the chloroform-acid/photometric method and in the presence of other bacterially derived pigments and metabolites. The proposed small and inexpensive sensor assembly is suggested for use in monitoring Ps. aeruginosa growth.

Item Type:Journal article
Keywords:Pyocyanin, bacteria, electrochemical, sensor
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:12597
Deposited By:Professor James Davis
Deposited On:25 Mar 2010 15:05
Last Modified:18 Aug 2011 11:18

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