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Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Erdem, A, Papakonstantinou, P, Murphy, H, McMullan, M, Karadeniz, H and Sharma, S (2010) Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection. Electroanalysis, 22 (6). pp. 611-617. [Journal article]

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URL: http://www3.interscience.wiley.com/journal/123308657/abstract?CRETRY=1&SRETRY=0

DOI: 10.1002/elan.200900436

Abstract

Disposable graphite pencil electrodes (PGE) modified with multiwalled carbon nanotubes (MWCNTs)-streptavidin (STR) conjugates were used for electrochemical monitoring of label-free DNA hybridization. The surface morphology of PGE electrode before and after hybridization was characterized by scanning electron microscopy. Electrochemical impedance spectroscopy was used to monitor each step of the construction of the DNA biosensor. The biosensor was demonstrated to have excellent selectivity, being able to differentiate complementary sequences from a noncomplementary ones and in addition select the target sequence of DNA from a mixture of other DNA without loss in current sensitivity.

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:12479
Deposited By:Professor Pagona Papakonstantinou
Deposited On:16 Mar 2010 15:16
Last Modified:18 Aug 2011 11:57

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