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Determination of total reduced thiol levels in plasma using a bromide substituted quinone

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Lobo, GAM, Chitre, SA, Rathod, SM, Smith, RB, Leslie, R, Livingstone, C and Davis, J (2007) Determination of total reduced thiol levels in plasma using a bromide substituted quinone. ELECTROANALYSIS, 19 (24). pp. 2523-2528. [Journal article]

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DOI: 10.1002/elan.200703977


The exploitation of 2-bromo-1,4-naphthoquinone (NQBr) as a selective redox label for the determination of reduced thiol functionalities (RSH) has been investigated. The system is selective for RSH functionality, giving distinct voltammetric signals for glutathione and cysteine but can also be adapted for broad spectrum thiol detection. Ion chromatographic protocols based on conductimetric detection enable total RSH analysis (protein and monomolecular moieties) within human plasma. Bromide released through the reaction can be easily quantified and integrated within normal IC measurements. The efficacy of the approach has been assessed and the response validated through comparison with the standard colorimetric technique.

Item Type:Journal article
Keywords:plasma; thiol; bromide; ion chromatography; oxidative stress
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:11904
Deposited By:Professor James Davis
Deposited On:14 Feb 2010 15:19
Last Modified:18 Aug 2011 11:18

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