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Bromide-sulfur interchange: Ion chromatographic determination of total reduced thiol levels in plasma

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Chitre, SA, Lobo, GAM, Rathod, SM, Smith, RB, Leslie, R, Livingstone, C and Davis, J (2008) Bromide-sulfur interchange: Ion chromatographic determination of total reduced thiol levels in plasma. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 864 (1-2). pp. 173-177. [Journal article]

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DOI: 10.1016/j.jchromb.2008.01.040

Abstract

Plasma thiol concentration has long been recognised as a potential indicator for assessing the severity of oxidative stress processes within physiological systems. While such measurements are normally restricted to research studies, this communication has sought to develop and characterise a novel approach through which this parameter could be exploited within routine clinical settings. The protocol is based on the rapid derivatisation of reduced thiol functionalities (protein and monomolecular moieties) through the homogenous reaction of a naphthoquinone bromide derivative. Bromide released in the reaction can be easily quantified through ion chromatography (Isocractic Dionex DX-120 incorporating an lonPac (R) AS 14 anion exchange column and a 25 mu L sample loop with conductivity detector. Mobile phase consisted sodium carbonate/bicarbonate (3.5 mM/1 mM) at a flow rate of 1.5 mL/min). Method selectivity and sensitivity has been critically evaluated. The technique covers the range 15 mu M-3.5 mM PSH with a detection limit of 9 mu M PSH and analysis time of 5 min. The efficacy of the approach for the analysis of human plasma from five volunteers was assessed (ranging from 49 to 72 mu M with an intra assay variation of less than 5% in all cases). The responses were validated through comparison with the standard Ellman colorimetric technique. (C) 2008 Elsevier B.V. All rights reserved.

Item Type:Journal article
Keywords:albumin; plasma; thiol; bromide; ion chromatography; oxidative stress
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:11902
Deposited By:Professor James Davis
Deposited On:14 Feb 2010 15:17
Last Modified:18 Aug 2011 11:18

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