Barnes, Clifford A, Elsaesser, Andreas, Arkusz, Joanna, Smok, Anna, Palus, Jadwiga, Lesniak, Anna, Salvati, Anna, Hanrahan, John P, de Jong, Wirn H, Dziubaltowska, Elzbieta, Stepnik, Maciej, Rydzynski, Konrad, McKerr, George, Lynch, Iseult, Dawson, Kenneth A and Howard, Vyvyan (2008) Reproducible Comet assay of amorphous silica nanoparticles detects no genotoxicity. NANO LETTERS, 8 (9). pp. 3069-3074. [Journal article]
Full text not available from this repository.
Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By using a carefully developed protocol and careful characterization of the nanoparticle dispersions, Comet assays were performed on 3T3-L1 fibroblasts with 3, 6, and 24 h incubations and 4 or 40 mu g/ml of silica nanoparticles. No significant genotoxicity was observed for the nanoparticles tested under the conditions described, and results were independently validated in two separate laboratories, showing that in vitro toxicity testing can be quantitatively reproducible.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences|
Faculty of Life and Health Sciences > School of Biomedical Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Nano Systems Biology
|Deposited By:||Dr G McKerr|
|Deposited On:||17 Feb 2010 11:10|
|Last Modified:||23 Jun 2011 16:20|
Repository Staff Only: item control page