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The characterisation of synthetic and natural-product pharmaceuticals by electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Smyth, Franklin, McClean, Stephen, Hack, Catherine, Ramachandran, VN, Doherty, B, Joyce, C, O'Donnell, F, Smyth, Thomas, O'Kane, E and Brooks, P (2006) The characterisation of synthetic and natural-product pharmaceuticals by electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 25 (6). pp. 572-582. [Journal article]

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DOI: 10.1016/j.trac.2006.04.003

Abstract

This article considers the application of topical analytical techniques-electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS - to the characterisation of selected small molecular-mass synthetic and natural-product pharmaceuticals, as well as bioactive peptides. We chose the synthetic drugs according to selected structural classes in which they give ESI signals primarily as [M + H](+) ions. The structural classes chosen to illustrate the application of ESI-MS and LC-ESI-MS to such drug characterisation are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, and 1,4-benzodiazepines and other heterocyclic hypnotics. We then discuss potential natural-product pharmaceuticals, such as selected quinolines and nicotines. We next apply the technique to the characterisation of molecules of unknown structure present in plant extracts or fractions that possess antibacterial activity. Finally, we discuss the isolation and the characterisation of bioactive peptides from frog-skin secretions utilising an arsenal of MS tools as well as Edman degradation sequencing and cDNA cloning. (c) 2006 Elsevier Ltd. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:1082
Deposited By:Prof W.Franklin Smyth
Deposited On:11 Jan 2010 16:40
Last Modified:22 Aug 2011 15:28

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